Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Diagnostic Performance and Clinical Relevance of CEBPD and GPD1 in MASH Patients. ( A ) Comparison of risk distributions between patients with MASH and healthy controls in the test set. ( B ) ROC curves assessed the model’s diagnostic performance in the test set. ROC curves assessed the diagnostic performance of CEBPD ( C ) and GPD1 ( D ) in the train set. ( E ) Comparison of CEBPD and GPD1 expression levels between patients with MASH and HC in the test set. ROC curves evaluated the diagnostic performance of CEBPD ( F ) and GPD1 ( G ) in the test set. ( H and I ) The association between the expression level of CEBPD and GPD1 and the fibrosis stage. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns not significant ( p ≥ 0.05).

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Diagnostic Assay, Comparison, Expressing

Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Immune Cell Profiling and Correlation with CEBPD and GPD1 Expression in MASH. ( A ) Heatmap showing correlations between 28 immune cell types in MerCorhort. ( B ) Heatmap showing the infiltrating enrichment of 28 immune cell types in MerCorhort. ( C ) Boxplot depicting differences in immune cell infiltration between patients with MASH and healthy controls. ( D ) Heatmap showing the correlation between CEBPD and GPD1 expression and immune cells in MASH. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns not significant ( p ≥ 0.05).

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Validation of GPD1 and CEBPD in a MASH Animal Model. ( A ) IHC staining of normal and MASH liver tissues, n = 6, 400×, scale bar 100 μm. ( B ) AOD values for GPD1 in CON and MASH groups. ( C ) AOD values for CEBPD in CON and MASH groups. ( D ) WB analysis of normal and MASH liver tissues, n = 3. ( E ) Relative gray value measurements for GPD1 in CON and MASH groups. ( F ) Relative gray value measurements for CEBPD in CON and MASH groups. *** p ≤ 0.001.**** p ≤ 0.0001.

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Biomarker Discovery, Animal Model, Immunohistochemistry

Journal: Cancer cell international

Article Title: TMEM71 is crucial for cell proliferation in lower-grade glioma and is linked to unfavorable prognosis.

doi: 10.1186/s12935-025-03747-5

Figure Lengend Snippet: Fig. 6 The expression and biological function of TMEM71 in LGG were verified in vitro. (A) Western blot and (B) qRT-PCR analysis of TMEM71 expression in NHA and LGG cell lines. (C) Wound healing assay to detect the migration and healing ability of sh-TMEM71 transfected and sh-NC transfected SW1783 and SW1088 cells. (D) The effect of sh-TMEM71 transfection on the cell viability of SW1783 and SW1088 cells was measured by CCK-8. (E) Effect of TMEM71 knockout on colony formation of SW1783 and SW1088 cells. (F) The invasive ability of SW1783 and SW1088 cells after sh-TMEM71 transfection and sh-NC transfection was detected by Transwell invasion assay. (G) TMEM71 gene set enrichment analysis (GSEA). (H, I) Western blot analysis of the effects of knock down (H) and overexpression (I) of TMEM71 on CEBPD expression in SW1783 and SW1088 cells. (J, K) Western blot analysis of the effects of knockdown (J) and overexpression (K) of TMEM71 on the expression of JAK2/STAT3 pathway marker protein in SW1783 and SW1088 cells. (L) Changes in JAK2/STAT3/ CEBPD protein levels after adding WP1006 to TMEM71 overexpressing cell lines

Article Snippet: Following this, the primary antibody TMEM71 (1:1000, ab235788, abcam, China), CEBPD (1:800, A15261, ABclonal, China), JAK2 (1:1000, A19629, ABclonal, China), Phospho-JAK2 (1:800, AP0531, ABclonal, China), STAT3 (1:1000, A1192, ABclonal, China) and Phospho-STAT3 (1:800, AP0705, ABclonal, China) were introduced and left to incubate at 4 °C.

Techniques: Expressing, In Vitro, Western Blot, Quantitative RT-PCR, Wound Healing Assay, Migration, Transfection, CCK-8 Assay, Knock-Out, Transwell Invasion Assay, Knockdown, Over Expression, Marker